PBMC Isolation using Lymphoprep
Principle of the separation procedure
This is a simple and effective protocol most commonly used to isolate mononuclear cells AKA PBMCs from human blood which was devised back in 1968.
The Lymphoprep Tube is a sterile tube in that contains the Lymphoprep (separation medium) under a plastic filter disc. This allow the PBS-diluted blood to be poured directly into the tube, while the filter disc prevents any mixing with the separation medium.
The separation principle is largely synonymous to the widely known density gradient centrifugation. On further elaboration, this procedure uses the strong centrifugal force to force components in solution to be “layered” according to their density. According to physics, the higher the density (heavier mass) of a component, the lower portion the component will be found in the tube.